Human Anti-VEGF Recombinant Antibody (clone B20-4) (CAT#: PABC-131)
Recombinant Human Antibody (B20-4) is capable of binding to VEGF, expressed in HEK293 cells. Expressed as the combination of a heavy chain (HC) containing VH from antiVEGF mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from antiVEGF mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody binds and blocks both human and murine vascular endothelial growth factor (VEGF).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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ELISA
Figure 1 binding of G6-31 and B20-4.1 as IgG (10 μg/ml) to ELISA wells coated with VEGF homologues is shown.
Liang, W. C., Wu, X., Peale, F. V., Lee, C. V., Meng, Y. G., Gutierrez, J., ... & Fuh, G. (2006). Cross-species vascular endothelial growth factor (VEGF)-blocking antibodies completely inhibit the growth of human tumor xenografts and measure the contribution of stromal VEGF. Journal of Biological Chemistry, 281(2), 951-961.
Specifications
- Immunogen
- Human Vascular endothelial growth factor
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human VEGF
- Species Reactivity
- Human, Mouse
- Clone
- B20-4
- Applications
- ELISA, Neut, Block
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Neutralization, Blocking.
Target
- Alternative Names
- Vascular endothelial growth factor; VEGF
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single Domain Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1036 | Recombinant Anti-VEGF Antibody | ELISA, WB, Dot, FuncS | IgG |
| MHH-1036 | Recombinant Human Anti-VEGF Antibody | ELISA, WB, FC, FuncS | IgG |
| HPAB-0746LY | Human Anti-VEGF Recombinant Antibody (HPAB-0746LY) | ELISA | Humanized IgG1 |
| HPAB-2806LY | Human Anti-VEGF Recombinant Antibody (HPAB-2806LY) | WB, FuncS | Humanized IgG |
| HPAB-2807LY | Human Anti-VEGF Recombinant Antibody (HPAB-2807LY) | ELISA, WB | Humanized IgG |
Customer Reviews and Q&As
Customer Review
Q&As
Michael Brown
Effective for Western Blots
Effective for Western Blots
The Anti-VEGF Recombinant Antibody (clone B20-4) has been a fantastic addition to our Western blot assays. Its specificity and sensitivity are top-notch, consistently providing clear results. This antibody has become essential for our experiments.
10.Mar,24
Jessica Davis
Essential for Immunoprecipitation
Essential for Immunoprecipitation
This antibody has been a key addition to our Western blot and immunoprecipitation assays. The detection of VEGF is always precise and clear, which has allowed us to produce consistent results without the need to worry about variability. Its performance has greatly enhanced our research quality.
18.May,23
David Martinez
Consistent in Flow Applications
Consistent in Flow Applications
We've used this antibody in several flow cytometry and immunofluorescence applications. The consistent high-quality results and strong affinity for VEGF make it a crucial part of our lab's toolkit. The reproducibility has been excellent, making our complex assays more reliable and effective.
27.Sep,22
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-131, RRID: AB_3111578)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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